Answer Happy

You and Ed review the protocol for generating a knockout mouse.Ed argues that the protocol will not allow you to study the role ofGene X in the trophoblast. His argument is that gene targetingoccurs in stem cells derived from the inner cell mass, nottrophoblast cells, and concludes that the trophoblast will notinclude the mutated gene.
a) How can you assure Ed that he can still use embryonic stemcells (ESCs) to ultimately affect the genes in the trophoblast?Describe the steps involved in this process and explain yourreasoning.
b) Would you use the chimeras you generated directly for yourexperiments? Why or why not?