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Question 27 CRISPR-Cas9 is a powerful technique for gene editing. Please place the stages of a CRISPR-cas9 gene editing workflow in the correct order below. Note, there is one incorrect, redundant answer. Please take care to answer this very carefully, there is no partial credit, you must have all the stages in the correct order to be awarded the marks for this question. Prompts Stage 1: Stage 2: Stage 3: Stage 4: Stage 5: Stage 6: Stage 7: Stage 8: Stage 9: Questions Filter (28) ▼ Submitted Answers Choose a match Choose a match Choose a match Choose a match Choose a match Choose a match Choose a match Choose a match 6 Points Choose a match ▼ ▼ ▼ ▼ O ● O O O Purify plasmids O Clonal characterisation (analysis of phenotype). Transfect cells O Sequence Synthesise DNA insert oligonucleotides Clone into CRISPR-cas9 expression vector Select for correctly gene edited cells (using antibiotic resistance and/or colour production for example) Design a set of targeting sequences using algorithms. Clonal isolation Add green fluorescent protein gene sequence into plasmid to aid selection of correctly transfected cells.