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1. List the four direct methods for determining bacterial numbers (Hint: lab manual). (2 points) 2. (Fill in the blanks) Counts by the Standard Plate Count (SPC) are reported as . (1 point) 3. Why is it necessary to perform a plate count in conjunction with the turbidimetry procedure? (2-3 sentences) (1 point) 4. If you transfer 1 mL of culture into a 99 mL of sterile water, then add 0.1 mL of that to an agar plate, what is the final dilution? (2 points) 5. If you transfer 0.1 mL of culture into a 99mL of sterile water, then add 1mL of that to an agar plate, what is the final dilution? (2 points) 6. (Fill in the blank) If you counted 225 colonies on a plate that received 1.0 ml of the 1:1,000,000 dilution then there are approximately. bacteria per milliliter in the original sample. (2 points)