Question 16 Choose the single most appropriate description of how most Next Generation sequencing methods work. A B D E
Posted: Thu Jul 07, 2022 12:45 pm
Question 16 Choose the single most appropriate description of how most Next Generation sequencing methods work. A B D E 3 Points Template DNA is attached to 'chips', free di-nucleotides are added to synthesise new DNA, computers read the results. Template DNA is attached to 'chips', di-deoxy nucleotides are added to synthesise new DNA, computers read the results. Sample DNA is broken into short fragments and synthetic oligonucleotides are added to them. They are bound to a membrane and sequenced using lasers. Sample DNA is broken into short fragments and synthetic oligonucleotides are added to them. The fragments are then bound to a membrane and sequenced using light emission as the reporter. Sequencing by synthesis of short template fragments, using various 'reporters' and computing power to rebuild lengthy sequence data in silico using reference genomes. F Sanger sequencing of short fragments, using various 'reporters' and computing power to rebuild lengthy sequence data in silico using reference genomes.