For an enzyme reaction followed spectrophotometricallyat 340 nm the Vmax was calculated at 0.14 ∆A340/min (ε340 = 5.22 m
Posted: Thu Jul 07, 2022 12:45 pm
For an enzyme reaction followed spectrophotometricallyat 340 nm the Vmax was calculated at 0.14 ∆A340/min (ε340 = 5.22 mM-1.cm-1). The 1 mL reaction in a 1 cm cuvette contained 30 ng of an enzyme (Mr of 100 000 g/mole). Calculate the Kcat for this enzyme. (6)