For an enzyme reaction followed spectrophotometrically at 340 nm the Vmax was calculated at 0.16 ∆A340/min (ε340 = 6.22
Posted: Thu Jul 07, 2022 12:45 pm
For an enzyme reaction followed spectrophotometrically at 340 nm the Vmax was calculated at 0.16 ∆A340/min (ε340 = 6.22 mM-1.cm-1). The 1 mL reaction in a 1 cm cuvette contained 30 ng of an enzyme (Mr of 100 000 g/mole). Calculate the Kcat for this enzyme.