Answer Happy

7. 6 points. A diagram of a gene called Tre1 is shown below. Normally, exons 1, 2, and 3 are present in the mature mRNA. Exon 1 Intron 1 Exon 2 Intron 2 A. A mutation occurred at the 5 splice site of intron 1 shown by the star, such that the spliceosome cannot bind to this location during mRNA processing of the pre-mRNA into the mature mRNA You use a Northern Blot to examine mRNA samples from an individual with this mutation, compared to an individual without this mutation (wildtype, WT)? What do you expect to see from the person who carries only the mutant allele of Tre1 shown above? Choose one of A through F and explain your choice in 1 sentence. Exon 3 WT A 8 C DE F
B. A mutant allele of a second gene, Thdaf2, causes histones in the region of the Tre1 gene to be acetylated more often compared to in a wildtype sample (Thdat2"). What would the Tref mRNA in the Thdat2- sample look like on the Northern Blot? Assume this sample has wildtype Tre 1. Choose one of A through F and explain your choice in 1 sentence.