Answer Happy

i think i got an answer for A and B so C is critical. Regardless, please try to answer. Genetics - Yeast Cloning and Sequencing Question 1) a) Was I successful at isolating plasmid DNA? Please explain b) Was I successful at isolating recombinant plasmid? Please explain c) If I was successful, what are the approximate sizes of the yeast fragments cloned DODELILI The first three lanes are loaded with the DNA Ladder, Undigested pUC19 and linear DUC19, respectively." Lane 4 is "ECORI - Digested purified plasmid Colony 1" and Lane 5 is "Eco-R1 -digested purified plasmid from colony #2
B DIDELIIII
1% TopVision LE CO Agarose @R0491) TITANS T bp ng/0.5µg % 10000 30.0 6.0 8000 30.0 6000 70.0 5000 30.0 4000 30.0 3500 30.0 3000 70.0 14.0 2500 25.0 5.0 2000 25.0 5.0 1500 25.0 5.0 1000 60.0 12.0 750 25.0 5.0 500 25.0 5.0 6.0 6.0 14.0 6.0 6.0 250 25.0 5.0 This is the expected band pattern and sizes of the DNA size marker used (GeneRulerTM 1 kb DNA ladder purchased from Fermentas). Some bands are brighter/thicker than others because they have more DNA. This can occur when the marker sample has multiple bands of approximately the same size. 0.5µg/lane, 8cm length gel, 1X TAE, 7V/cm, 45min Figure 2. Expected band pattern and sizes of GeneRuler™M 1 kb DNA ladder.
DNA Size Marker Undigested PUC 19 DeP/EcoRI/ PUC19 Group #1 samples Group #2 samples
1% TopVision" LE GQ Agarose #R0491) - - - - bp ng/0.5ug % 10000 30.0 8000 30.0 6000 70.0 14.0 5000 30.0 6.0 4000 30.0 6.0 6.0 6.0 3500 30.0 6.0 3000 70.0 14.0 2500 25.0 2000 25.0 1500 25.0 0.5µg/lane, 8cm length gel, 1X TAE, 7V/cm, 45min 5.0 5.0 5.0 1000 60.0 12.0 750 25.0 5.0 500 25.0 5.0 250 25.0 5.0