You are studying a new enzyme and want to determine Km and Vmax. You collect your initial rate data at multiple substrat
Posted: Thu Jul 07, 2022 12:44 pm
You are studying a new enzyme and want to determine Km and Vmax.You collect your initial rate data at multiple substrateconcentrations, take the reciprocal of v and s, and do aLineweaver-Burke plot. If the slope of the resulting line is 0.0152micromolar minutes and the y-intercept is 0.0052 per min, what isthe Km of this enzyme for its substrate? Report your answer inmicromolar to the nearest 0.1 uM.