Problem Your friends have successfully produced Cobalamin (Vitamin B12) using Propionibacterium shermanii culture in 250

[answerhappygod]

Problem Your Friends Have Successfully Produced Cobalamin Vitamin B12 Using Propionibacterium Shermanii Culture In 250 1 (81.53 KiB) Viewed 39 times

Problem Your friends have successfully produced Cobalamin (Vitamin B12) using Propionibacterium shermanii culture in 250 mL Erlenmeyer flask. You want to try producing Cobalamin using their bacteria culture in your own laboratory, using their optimized method, below 1) Propionibacterium shermanii is grown in 150 mL Nutrient Broth + 2% glucose (in 250 mL Erlenmeyer flask) for 3 days in room temperature, anaerobically. The initial concentration of the culture is 250 – 300 x 106 CFU/mL 2) Afterward, the fermentation is continued for another 3 days with gentle aeration Questions: a) How do you adjust/arrange that the concentration of the culture is 250 – 300 x 106 CFU/mL in the beginning of the fermentation? b) How do you conduct/give gentle aeration to your culture start from day 4, with minimum risk of contamination?