1. All agonists acting on constitutive receptors can mediate inverse agonists and negative efficacy. a) True b) Fa
Posted: Wed Jul 06, 2022 10:34 am
1. All agonists acting on constitutive receptors can mediateinverse agonists and negative efficacy.
a) True b) False
2. Neutral antagonists can block (inhibit) bothagonist- and inverse agonist-mediated responses.
a) True b) False
3. Functional or activity assays can often be moresensitive than binding assays due to amplification of signallingpathways.
a) True b) False
4. You may choose more than one answer. Which of thefollowing might be a problem when using cells in culture forfunctional drug screening?
a) Adaption of cells for culture can lead to changes inprotein expression within the cell which can affect signallingpathway activation and produce variable functional responsesbetween different cell lines.
b) Variable G protein alpha subunit expression can producevariable functional responses.
c) Cells with a slow proliferation rate will be negativelyselected.
d) Asynchronous cell populations may represent varying functionsdependent on cell cycle.
5. You may choose more than one answer. The finding that"different receptor active conformations trigger portions of thecell repertoire of behaviours but not necessarily all of them":
a) has been observed with GPCR.
b) has been called ‘collateral efficacy’.
c) suggests that more than two different conformations of areceptor exist.
d) is not a valid finding.
6) Choose the best, and most complete, answer.
NMDA receptors are modulated by which combination of signalsbelow?
a) glutamine, glycine
b) gaba, glycine
c) glutamate, glycine, depolarization
d) glutamine
7) Using recombinant technology, you transfect thecDNA corresponding to a human cell surface receptor into a CHOcell line. The function of the receptor appears different comparedto that in the human cells. A possible reason(s) for thisinclude:
a) different expression levels
b) different folding and/or conformation of the receptor
c) different post-translational modifications to thereceptor protein
d) None of the above
8) Cocaine inhibits the NET(norepinephrine transporter). What impact would cocaine haveon cholinergic transmission?
a)Increase levels of transmission
b) Decrease levels of transmission
c) No effect
9. You may choose more than one answer. Which ofthe following are TRUE:
a) Ionotropic receptors acts on channels which open andclose quickly.
b) G protein coupled receptors are examples ofionotropic receptors.
c) Metabotropic receptors mediate slower responsesthan ionotropic receptors because theytransmit signals via G proteins or otheradaptor proteins.
d) The types of ligands that bind tometabotropic receptors include adrenaline, ATP andglutamate.
10. You may choose more than oneanswer. Serotonin activates what type(s) ofreceptors?
a) G protein coupled receptors
b) Direct kinase activity receptors
c) Intracellular enzymes
d) Ligand-gated ion channels
a) True b) False
2. Neutral antagonists can block (inhibit) bothagonist- and inverse agonist-mediated responses.
a) True b) False
3. Functional or activity assays can often be moresensitive than binding assays due to amplification of signallingpathways.
a) True b) False
4. You may choose more than one answer. Which of thefollowing might be a problem when using cells in culture forfunctional drug screening?
a) Adaption of cells for culture can lead to changes inprotein expression within the cell which can affect signallingpathway activation and produce variable functional responsesbetween different cell lines.
b) Variable G protein alpha subunit expression can producevariable functional responses.
c) Cells with a slow proliferation rate will be negativelyselected.
d) Asynchronous cell populations may represent varying functionsdependent on cell cycle.
5. You may choose more than one answer. The finding that"different receptor active conformations trigger portions of thecell repertoire of behaviours but not necessarily all of them":
a) has been observed with GPCR.
b) has been called ‘collateral efficacy’.
c) suggests that more than two different conformations of areceptor exist.
d) is not a valid finding.
6) Choose the best, and most complete, answer.
NMDA receptors are modulated by which combination of signalsbelow?
a) glutamine, glycine
b) gaba, glycine
c) glutamate, glycine, depolarization
d) glutamine
7) Using recombinant technology, you transfect thecDNA corresponding to a human cell surface receptor into a CHOcell line. The function of the receptor appears different comparedto that in the human cells. A possible reason(s) for thisinclude:
a) different expression levels
b) different folding and/or conformation of the receptor
c) different post-translational modifications to thereceptor protein
d) None of the above
8) Cocaine inhibits the NET(norepinephrine transporter). What impact would cocaine haveon cholinergic transmission?
a)Increase levels of transmission
b) Decrease levels of transmission
c) No effect
9. You may choose more than one answer. Which ofthe following are TRUE:
a) Ionotropic receptors acts on channels which open andclose quickly.
b) G protein coupled receptors are examples ofionotropic receptors.
c) Metabotropic receptors mediate slower responsesthan ionotropic receptors because theytransmit signals via G proteins or otheradaptor proteins.
d) The types of ligands that bind tometabotropic receptors include adrenaline, ATP andglutamate.
10. You may choose more than oneanswer. Serotonin activates what type(s) ofreceptors?
a) G protein coupled receptors
b) Direct kinase activity receptors
c) Intracellular enzymes
d) Ligand-gated ion channels