1. You have learned how to set up a restriction endonuclease reaction to cut DNA. Suppose you are told to cut two microg
Posted: Wed Jul 06, 2022 10:34 am
1. You have learned how to set up a restriction endonucleasereaction to cut DNA. Suppose you are told to cut two micrograms(2g) of plasmid DNA with the restriction enzyme ApeKI in areaction volume of 50 microliters (50l). Go to www.neb.com tolearn more about this enzyme
A) Write below the two “pieces” of DNA that result when thissequence has been cut with ApeKI. Show the 5’ ends of each strandon each piece of DNA:
B) Does this enzyme generate 5’ overhangs, 3’ overhangs, or doesit generate blunt ends?
C) This enzyme is usually employed with a buffer called NEBuffer3.1. In lab, you often use something called CutSmart® buffer.Referring to the www.neb.com website, which provides better cuttingconditions for the ApeKI enzyme please explain how much better isthat buffer than the other?
A) Write below the two “pieces” of DNA that result when thissequence has been cut with ApeKI. Show the 5’ ends of each strandon each piece of DNA:
B) Does this enzyme generate 5’ overhangs, 3’ overhangs, or doesit generate blunt ends?
C) This enzyme is usually employed with a buffer called NEBuffer3.1. In lab, you often use something called CutSmart® buffer.Referring to the www.neb.com website, which provides better cuttingconditions for the ApeKI enzyme please explain how much better isthat buffer than the other?