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Painter BIU abcx, x' A--A- words KIP ype here to search Font WA.. Paragraph AiRbCcDX AaBbCebe AaBbC AaBbCe 1 Normal 1 No Spac... Heading 1 Heading 2 Identification of an unknown quiz You will be using information and techniques you have learned to identify an unknown. In the real world, identification of a bacteria requires a number of biochemical tests and media (or genetic methods): But since we are going to narrow it down to a few bacteria that differ in at least one characteristic, we will be able to do this. This quiz is worth 50 points, so be very thorough in your answers. You will need the Table of Bactens to complete this assignment. 1. You have been provided with a broth culture containing an unknown (the unknown will come from the table). The instructor tells you that the broth culture is pure, but since so much of your grade is riding on this, you want to make sure. What would be the first thing you do to obtain a pure culture, and why? (4 points) 2. Below is an image of your unknown growing on TSA, (5 points) B a. Describe the growth of your unknown on TSA, using information you learned in Lab 4. Make sure to provide enough information to help you determine what unknown you have. Came
my man Epic Generic Find- Replace aB AaBbcck AaBbCcD AaBbCcD AaBbCcD AnhCD AgBbCD AaBbCED, AABBCCDC AABICCOC AoBbCcA Quote Intense Q. Subtle Re Intense Re Book Title Select- tle Subtitle Subtle Em Emphasis Intense E Strong Eating 3. Below are your Gram stain results. (8 points) Styles a. What does this result tell you (consider gram reaction, shape, morphology? Unknown b. Using your Table of Bacteria, which bacteria can you exclude? Explain your answer, and list your evidence. (Please provide the specific names of the bacteria you can cross off your list.) 3. You determine the temperature requirements of your unknown, and get the following results **means no growth; "/" means there may be slight growth; means slight amount of growth means moderate amount of growth and +++ means heavy growth. (9 points) Bacteria 4C refrigerator 10C 25C 37C 55C *** a. Is this bacteria a psychrophile, psychrotroph, mesophile, or thermophile? How do you know? b. Based on your results, what is the optimum temperature of your bacteria? c.. Based on this answer, are there any other bacteria you can exclude? Why, or why not? G B5EAM
AaBbceD: AaBbCeD. AaBbC AaBuce AaB AaBbcct AgBUCcDN AaBbCcD AGBbCcD AaBbce Normal No Spac... Heading 1 Heading 2 Title Subtitle Subtle Em... Emphasis intense E... Stronge Styles 4. You determine the oxygen requirements of your unknown bacteria and receive the following results. (12 points) 3. If we are studying oxygen requirements, what media is this? b. After you inoculate your media, what temperature would you use to incubate it? Why? What do these results tell you about your bacteria, in terms of oxygen requirement? d. Does this result help you narrow down your bacteria? Why or why not? 5. What is the identity of your unknown? Please list and explain all lines of evidence that allowed you to determine the identity of your unknown. You need to be thorough in your answer. (10 points) 6. Were there any tests that did not need to be done based on your Gram stain results? Which media did not have to be used, and why? (2 points)