Given the following sequence, what would be a possible gRNA (on either strand) for Cas9 (Cas9 recognizes 'NGG' PAM seque
Posted: Wed Jul 06, 2022 10:22 am
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Given the following sequence, what would be a possible gRNA (on either strand) for Cas9 (Cas9 recognizes _GG' PAM sequences, where 'N' can be any nucleotide). 5'-TTGAAAGCTGTGTCTGTAAACTGATGGCT-3' 5'-GTCATCCTAGGCATATCGGG|AGG-3' TARGET 5'-AAGCTGTGTCTGTAAACTGA|TGG-3' TARGET | PAM TARGET 5'-AGTTCAGTCAAGTTTGCCTG|AGG-3' IPAM TARGET IPAM 5'-AGTTCAGTCAAGTTTGCCTG| AGG-3¹ IPAM
Given the following sequence, what would be a possible gRNA (on either strand) for Cas9 (Cas9 recognizes _GG' PAM sequences, where 'N' can be any nucleotide). 5'-TACCTAGATGTTTTAACAGAAAAAGAAATAT-3¹ 5'-AGTTCAGTCAAGTTTGCCTG|AGG-3' TARGET 5'-AGTTCAGTCAAGTTTGCCTG|AGG-3¹ TARGET IPAM TARGET Ⓒ5-CTTTTTCTGTTAAAACATCT | AGG-3' |PAM TARGET I PAM 5'-GTCATCCTAGGCATATCGGG|AGG-3¹ IPAM