20 g of sediment are resuspended in 40 mL of saline solution and vigorously shaken. Then, the suspension is sonicated to

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answerhappygod
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20 g of sediment are resuspended in 40 mL of saline solution and vigorously shaken. Then, the suspension is sonicated to

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20 g of sediment are resuspended in 40 mL of saline solution andvigorously shaken. Then, the suspension is sonicated to separatethe bacteria from sediment particles and 10 mL are centrifuged atlow speed (sedimentation of particles > 3 mm). The resultantsupernatant is diluted 1,000 times and 0.2 mL are plated onnutrient agar. After incubation, 126 colonies were present on theplate. How many culturable bacteria were present per g ofsediment?
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