Genetics - Yeast Cloning and Sequencing Question 1) a) b) Was I successful at isolating recombinant plasmid? Please expl
Posted: Tue Jul 05, 2022 7:44 am
Genetics - Yeast Cloning and Sequencing
Question 1)
a)
b) Was I successful at isolating recombinant plasmid? Please explain
The first three lanes are loaded with the DNA Ladder, Undigested pUC19 and linear pUC19, respectively.* Lane 4 is "ECORI - Digested purified plasmid Colony 1" and Lane 5 is "Eco-R1 - digested purified plasmid from colony #2"
1 B DODELITI
DNA Size Marker Undigested pUC 19 DeP/EcoRI/ pUC19 Group #1 samples Group #2 samples
Question 1)
a)
b) Was I successful at isolating recombinant plasmid? Please explain
The first three lanes are loaded with the DNA Ladder, Undigested pUC19 and linear pUC19, respectively.* Lane 4 is "ECORI - Digested purified plasmid Colony 1" and Lane 5 is "Eco-R1 - digested purified plasmid from colony #2"
1 B DODELITI
DNA Size Marker Undigested pUC 19 DeP/EcoRI/ pUC19 Group #1 samples Group #2 samples