Answer Happy

2. You run a 2D gel and get the result shown. You notice pairs of spots that are on the same horizontal level (horizontal arrows), but also two that are roughly the same size and near, but not exactly on, a vertical line with each other (angled arrows). Which technique was used to vertically separate the spots shown by the angled arrows, and what is their most likely relationship to each other? A. IEF, phosphorylated vs unphosphorylated B. IEF, RNA splicing variations of the same gene C. SDS, phosphorylated vs unphosphorylated D. SDS, unrelated proteins