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* Obtain a sterile glass microscope slide. With a wax pencil, draw a line that divides the slide into left and right sides. Label the left side "A" and the right side "B" Obtain a second sterile glass microscope slide and label it "Rh." Place a paper towel on your work space. This towel will be used to place blood-collecting instruments prior to disposal Wash your hands thoroughly with warm water and soap and dry them completely with a paper towel. You will be collecting a blood sample from yourself. Put on protective eyewear and a face mask. Place a surgical glove on the hand that will hold the lancet, but not on the hand from which you will collect the sample. Use a sterile alcohol pad to clean the tip of your index finger on all sides. Place the used pad on the paper towel Anti-A Type AB Anti-B Agglutination O Examine the blood samples for evidence of agglutination. Agglutination will occur when i antibodies in the antiserum react with the corresponding surface antigen on red blood cells. Record your ABO blood type: Anti-A Type B Anti-B Open a sterile blood lancet to expose the sharp tip. Quickly jab the lancet tip into your fingertip, and then place the lancet on the paper towel. Never use the same lancet more than once. Squeeze a drop of blood onto each half of the slide labeled A and B, and a third drop onto the slide labeled Rh. Add a drop of anti-A serum to blood sample A. Do not touch the blood samples with the dropper. In the same way, add a drop of anti-B serum to blood sample B and a drop of anti-D serum to blood sample Rh. Mix the blood samples and antisera with clean mixing sticks. Use a new, clean stick to mix each sample. Place the used mixing sticks on the paper towel. Place both slides on a warming tray. Gently agitate the samples back and forth for 2 minutes. Anti-A Type A Rh+ blood will agglutinate when exposed to anti-D serum, but Rh- blood will not. Rh agglutination is often difficult to observe with the unaided eye. If that is the case, use a microscope to observe the reaction. Record your Rh status: Anti-B Anti-A Type Of Anti-B No agglutination 10 Assess the Outcome What is your blood type? Did your result agree with your prediction? Explain. MAKING CONNECTIONS While typing the blood of a patient who is about to undergo surgery, the medical technician determines that agglutination occurs when the blood is exposed to both anti-A and anti-B antibodies. Agglutination does not occur when the blood is exposed to anti-D antibodies. What is the patient's blood type? Explain. apenancomwita Sarto win>PhysioEx>Exercise 11: Blood Analysis > Activity 4: Blood Typing 367