Answer Happy

3. For each of the following scenarios, indicate whether it is an example of forward or reverse genetics and why (8 points): a. You've discovered a new species of butterfly and want to know whether Hox genes are involved in specifying the head-to-tail body plan during embryonic development, a role they are known to play in many other animals. b. You want to know what other genes are also involved in embryonic development process, so you mutagenize a population of the butterflies and examine their embryos to find ones whose body plan is disrupted. c. In trying to determine which genes are involved in the alignment of chromosomes at the metaphase plate during mitosis, you search a mutant library for cells that fail to align their chromosomes. d. You suspect that the gene Nuf2p, which encodes a protein that helps microtubules attach to chromosomes during cell division, is important for this process, so you knock down the gene using RNAi and observe the cells' phenotype.