Answer Happy

What is the correct sequence of steps in a Polymerase Chain Reaction protocol? A. Annealing - Denaturation - Polymerization B. Denaturation - Polymerization - Annealing C. Annealing - Polymerization - Denaturation D. Denaturation - Annealing - Polymerization E. Polymerization - Denaturation - Annealing Your colleague has run a PCR of DNA samples from the new mouse line you recently got in the lab. He shows you the results and you see a mixture of non-specific products. What could have happened? A. He forgot to put dNTPs B. He forgot to put Taq polymerase C. He forgot to put dNTPs, primers and Taq polymerase D. He used shorter and variable primers E. He used not all dNTPs 5. You decide to replicate the same gel electrophoresis on the samples your colleague used. Once you look at the gel on the UV light you still find many non-specific products. You are sure you used the correct primers this time. What could have happened? A. You put twice the amount of dNTPs B. You put twice the amount of Taq polymerase C. You used twice the amount of primers D. The PCR temperature during polymerization was too high E. The PCR temperature during annealing was too low