Answer Happy

is this right? im not positive if it is
Gram Stain Results: a red bacillus Catalase Test Results Triple Sugar Iron Agar Test Results, red slant, yellow butt, and bumping obscured by precipitate ASM MicrobeLibrary Capsule Stain Results: a bacillus with no halo Starch hydrolysis results after the addition of iodine. Lysine Iron Agar Test Results precipitate obscures purple slant and butt Endospore Stain: Malachite green and safranin are used to stain bacilli DNA Hydrolysis test with Dnase Agar containing methyl green. MacConkey Agar after inoculation and opportunity for growth. Positive Convol Sample Gelatinase test, sample is on bottom and positive control is on the top. Tubes are held near parallel to the floor. Mannitol Salts Agar after inoculation and opportunity for growth. Citrate utilization test Eosin Methylene Blue agar after inoculation and opportunity for growth. Sample Sterile Control Urea Hydrolysis test. Sample on left, uninoculated control on right

Organism Bacillus cereus Escherichia coli Klebsiella pneumoniae Micrococcus luteus Pseudomonas aeruginosa Proteus vulgaris Staphylococcus aureus Salmonella typhimurium Bacillus Cell Morphology Gram Stain Capsule Stain Bacillus Negative Bacillus cocci Bacillus Positive Cocci Serratia marcescens Bacillus Bacillus Negative Positive Microscopy Cell Stains Negative Negative Positive Negative Positive Negative Positive Negative Positive Negative Endospore Stain Negative Positive Positive Negative Negative Negative Negative Negative Negative Negative Acid-fast Stain MacConkey Agar Negative Negative Negative Lactose Fermenter Negative Bacillus Negative Negative Negative Negative Negative Negative Negative No Growth Negative Differential & Selective Media Lactose Fermenter No Growth Lactose Non- fermenter Lactose Non- fermenter No Growth Lactose Non- fermenter Lactose Non- fermenter Mannitol Salts Agar Non-fermenter No Growth No Growth Non-fermenter No Growth No Growth Ferments Mannintol No Growth No Growth Eosin Methylene Blue Agar No Growth Ferments Lactose and/or sucrose Ferments lactose and/or surcose No Growth Growth, Colorless colonies Growth, Colorless colonies No Growth No Growth Growth, Colorless colonies Catalase Test Starch Hydrolysis Positive Positive Positive Positive Positive Positive Positive Positive Culture-based Methods Positive Positive Negative Positive Negative Negative Negative Negative Negative Negative Gelatin DNA Citrate Hydrolysis Hydrolysis Utilization Positive Biochemical Tests Negative Positive Negative Negative Negative Negative Negative Positive Negative Positive Positive Negative positive Positive positive Positive Positive Negative Positive Negative Positive Positive Positive Positive Urea Hydrolysis Positive Ngative negative Positive Variable Positive(Strong) variable Negative Positive(Strong) Negative TSIA K/A A/A, G A/A A/A K/K A/A, G KA LIA R/A K/K K/K K/K R/A R/A K/K Negative K/A, H₂S, G K/K, H₂S

Is this organism gram positive or gram negative? Gram negative Positive for capsule prod Is this organism positive or negative for growth on MacConkey Agar? Negative for growth on V Is this organism positive or negative for lactose fermentation while growing on MacConkey Agar? Select negative if the organism did not grow on MacConkey Negative for lactose fern Is this organism positive or negative for growth on Mannitol Salts Agar? Negative for growth on ✓ Is this organism positive or negative for capsule production? Is this organism positive or negative for endospore production? Negative for endospore Is the organism positive or negative for fermentation of Mannitol while growing on Mannitol Salts Agar? Select negative if the organism did not grow on Manni Agar. Positive for mannitol fer ✓ Is this organism positive or negative for growth on Eosin Methylene Blue agar? Positive for growth on Et V Is the organism positive, positive: vigorous fermentation, or negative t fermentation of lactose while growing on EMB Agar? For this question only, choose be positive, negative, or positive: vigorous fermentation. Select negative if the organism did not grow on EMB agar. Positive, but fermentatio V Was the organism positive or negative for the catalase test? Negative for catalase act ✓ Negative for DNase activ Was the organism positive or negative for starch hydrolysis? Positive for starch hydro ✓ Negative for urea hydrol Was the organism positive or negative for DNA Hydrolysis? Was the organism positive or negative for Gelatinase? Negative for gelatinase a Was the organism positive or negative for Citrate Utilization? Positive for citrate utiliza ✓ Was the organism positive or negative for Urea Hydrolysis? How would you score this organism for Lysine Iron Agar? K/K How would you score this organism for Triple Sugar Iron Agar? K/A Using the table of expected results below, how would you identify this unknown organism? choose your answer...