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Conclusions: Effect of Temperature The bubble column height indicates the degree of enzyme activity. Was your bypothesis supported? yes •What is your conclusion concerning the effect of temperature on entyme activity? Explain in Table 5.2 the degree of enzyme activity per tube Laboratory Manual to accompany Biology, 14th Edition 5.3 Effect of Concentration on Enzyme Activity Consider that if you increase the number of caretakers per number of children, it is more likely that each child will have quality time with a caretaker. So it is if you increase the amount of enzyme per amount of substrate It is more likely that substrates will find the active site of an enzyme and a reaction will take place. With this in mind, examine Table 5.3 and hypothesize which tube (1, 2, or 3) will have the most product per unit time as judged by bubble column height. Explain your answer.. Experimental Procedure: Effect of Enzyme Concentration Number three clean test tubes and use the appropriate transfer pipet to add solutions to the test tubes as follows: 1. To each tube, add 4 ml of hydrogen peroxide. 2. To tube 1, add 1 ml of water. 3. To tube 2, add 1 ml of buffered catalase. 4. To tube 3, add 3 ml of buffered catalase. 5. Swirl well to mix before measuring the height of the bubble column. 6. Measure the height of the bubble columns, and record your results in Table 5.3. Table 5.3 Effect of Enzyme Concentration Tube Amount of Enzyme 1 2 none Bubble Column Height (mm) Explanation Conclusions: Effect of Concentration The bubble column height indicates the degree of enzyme activity. Was your hypothesis Explain in Table 5.3 the degree of enzyme activity per tube supported? .If unlimited time were allotted, would the results be the same in all tubes? Explain why or why not. Would you expect similar results if the substrate concentration were varied in the same manner as the enzyme concentration? Why or why not? • What is your conclusion concerning the effect of concentration on enzyme activity? MacBook Air 65 Laborary 5 How Eyes Fundo 63