Answer Happy

Run kinetics experiments on XYZase without inhibitors and with Compound X. Results: The compound found in Dr. Bleu's coffee (Compound X) has yet to be identified, but using the small amount extracted, you have discovered that it is an inhibitor of XYZase. Given this revelation, it seems clear that Compound X is the most likely murder weapon. The data you have gathered is shown in Table 1. Table 1 [S] (nM) 50 75 150 400 Vo (no inhibitor) (µmol) 0.14 0.19 0.32 0.47 Vo (Compound X) (μmol) 0.040 0.050 0.080 0.14

Compare Samples with and without Compound X. Results: A Lineweaver-Burk plot made from the data with and without Compound X is shown in Graph 2. Lineweaver-Burk plot 1/V +0.005 25 20 15 10 No inhibitor 0.005 0.01 0.015 0.02 y= 291x + 1.32 1/[S] ■ Compound X ********** Graph 2 y = 1023x + 5.29

Interpret graphs. Results: The conclusions you have made from the graph of Compound X and the samples collected from the labs is shown in Table 3. Table 3 Inhibitor Compound X Sample 1 Sample 2 Sample 3 Table 4 Km no effect increase no effect decrease Researcher Dr. Gray Dr. Greene Dr. Wight V Interview Researchers. Results: The conclusions you have made from the interviews are shown in Table 4. Km increase decrease no effect max decrease no effect decrease decrease Vmax no effect Inhibitor Type ? competitive noncompetitive uncompetitive decrease decrease Inhibitor Type competitive uncompetitve irreversible

Based on their kinetic mechanisms of inhibition, which sample matches Compound X? O Sample 1 O Sample 2 O Sample 3

You now have reason to believe that Compound X is one of the three inhibitor samples that you tested from Hinesbiopharma. What type of inhibitor is Compound X? O competitive O unncompetitive O noncompetitive irreversible

Who murdered Dr. Bleu? Dr. Wight Mrs. Bleu Dr. Gray Dr. Greene