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Fill in the tables with your data D Table 1: Viable and Direct Counts Plate set counted (B, C, D or E) Colonies counted
Posted: Thu Jun 30, 2022 6:35 pm
by answerhappygod
- Fill In The Tables With Your Data D Table 1 Viable And Direct Counts Plate Set Counted B C D Or E Colonies Counted 1 (64.5 KiB) Viewed 60 times
Fill in the tables with your data D Table 1: Viable and Direct Counts Plate set counted (B, C, D or E) Colonies counted Table 2. Plate count calculations Notes: B C D Tube/Bottle Original Culture (OC) A E (OC) ½ . Original Culture ½ . ½ 1/16 0.01 0.01 0.1 0.1 SD and TD can be filled in on day one. The concentration of the tube corresponding to the plate you counted is calculated by dividing the average colonies on the plate by the amount plated or 0.1ml. The concentration in the OC can be calculated by dividing the conc. In the tube corresponding to the plate counted (what you did above) by the total dilution at that tube. The concentration in the other tubes can be calculated by multiplying the TD at that tube by the conc. Of the OC Serial Dilution Total Dilution 04 Average: Add it up + 3 = Replicate 1 Replicate 2 Replicate 3 Average 02 D3 147 15 145 39 Table 3. Total and Indirect Counts Cuvette Percent OD Culture concentration transmittance = 2-log of perder=0.D x 1.25 x 10% 60 78 0.01 0.0001 0.00001 0.000001 0.0000001 1.40 0.82 142 0.41 0.22 144.6 0.11 Concentration (Cells/ml) Homework 2 Fill it in' Cells/mi