Consider a second experiment: Graduate students repeated the above experiment with one modification. The same protocol w
Posted: Thu Jun 30, 2022 6:35 pm
Consider a second experiment: Graduate students repeated the above experiment with one modification. The same protocol was used for the LB + ampicillin plate, plating 100 microliters of the reaction mix directly to the LB + amp plate. However, they modified the protocol for the LB only plate by diluting the transformation mix by 1/1000 before plating. They then plated 100 microliters of this 1/1000 dilution on the LB only plate. When viewing the colonies (after 2 days of growth), they obtained the following results: LB + ampicillin plate: 175 colonies LB only plate: 50 colonies From this data, calculate the percentage of cells transformed in this experiment. Select one: O a. 0.0035% O b. 0.35% O c. 3.5% O d. 0.29% e. 28.6%