Answer Happy

Imagine you are trying to express your favorite protein (YFP encoded by the YFG gene) from Drosophila melanogaster in E. coli. You have a plasmid including a complete YFG open reading frame from YFG cDNA, but you need to insert the ORF into an expression vector. Here is the part of the plasmid that includes the YFG ORF: EH 艹 20 40 B H 550 Promoter 200 A 100 50 YFG cDNA Below is the expression vector into which you are trying to clone the ORF. Numbers are distances in bp between the restriction sites and restriction sites are for BamHI, HindIll, EcoRI, and Acvl. The promoter and terminator are from E. coli and amp' confers ampicillin resistance in E. coli. B EH 3000 800 3550 bp total Terminator 200 H ampr EH 300 20 Which enzyme or combination of enzymes would be the best choice for cloning the YFG ORF into your cloning vector to give the best chance that the protein will be expressed?