https://www.labxchange.org/library/items/lb:LabXchange:b4d9f467:lx_simulation:1Links to an external site. Work through e
Posted: Thu Jun 30, 2022 6:34 pm
https://www.labxchange.org/library/item ... n:1Linksto an external site.
Work through each of the components and answer the followingquestions. You may also use other sources (e.g. lecture notes) tohelp you with your answers. Use your own words.
1. What is the difference between a chromosome and aplasmid?2. What is "transformation"?3. What is heat shock? Why do cells have to recover for some timeafter? How is this done?4. What medium is used for growing transformed cells?5. What is meant with "some plasmids carry anampR gene"?6. What are competent cells?7. Why do cells have to be pre-treated with calcium choride?8. Why is it important to include controls?9. Go to the predictions. Answer the two questions and enter thenumbers of the tubes here.10. Summarize the transformation experiment. What was down and how?Why was aseptic technique so important when handling thesesamples?11. Perform the experiment. Where your experimental resultscorrect? Submit a screenshot.
Work through each of the components and answer the followingquestions. You may also use other sources (e.g. lecture notes) tohelp you with your answers. Use your own words.
1. What is the difference between a chromosome and aplasmid?2. What is "transformation"?3. What is heat shock? Why do cells have to recover for some timeafter? How is this done?4. What medium is used for growing transformed cells?5. What is meant with "some plasmids carry anampR gene"?6. What are competent cells?7. Why do cells have to be pre-treated with calcium choride?8. Why is it important to include controls?9. Go to the predictions. Answer the two questions and enter thenumbers of the tubes here.10. Summarize the transformation experiment. What was down and how?Why was aseptic technique so important when handling thesesamples?11. Perform the experiment. Where your experimental resultscorrect? Submit a screenshot.