Bovine serum albumin is often used as a concentration standard for proteins. Densitometric measurement of BSA bands on a
Posted: Thu Jun 30, 2022 6:14 pm
Bovine serum albumin is often used as a concentration standardfor proteins. Densitometricmeasurement of BSA bands on an SDS-PAGE gel gave the followingvalues for differentconcentrations of BSA (plotted below and shown in table):
A solution of protein Z is run on three lanes of a gel atdifferent concentrations. The lane withundiluted solution gave a densitometry measurement of 75 counts.The other lanescorresponding to 0.20 and 0.50 mL of the solution were diluted to1.0 mL, and gave counts of14 and 36, respectively.What is the average value in μg/mL for the concentration of proteinZ in the undiluted solution?
Counts (a.u.) 120 100 80 40 20 0 0 1 BSA Standard Curve 2 3 BSA Concentration (µg/mL) BSA concentration (µg/mL) 0.5 1.0 2.0 3.0 4.0 Counts (a.u.) 14 28 54 81 111 y = 27.463x R² = 0.9993 4 5
A solution of protein Z is run on three lanes of a gel atdifferent concentrations. The lane withundiluted solution gave a densitometry measurement of 75 counts.The other lanescorresponding to 0.20 and 0.50 mL of the solution were diluted to1.0 mL, and gave counts of14 and 36, respectively.What is the average value in μg/mL for the concentration of proteinZ in the undiluted solution?
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