The Toolkit available enzymes otrypsin otrypsinogen chymotrypsin chymotrypsinogen O enteropeptidase prophospholipase A2

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The Toolkit Available Enzymes Otrypsin Otrypsinogen Chymotrypsin Chymotrypsinogen O Enteropeptidase Prophospholipase A2 1 (116.99 KiB) Viewed 11 times

The Toolkit available enzymes otrypsin otrypsinogen chymotrypsin chymotrypsinogen O enteropeptidase prophospholipase A2 phospholipase A2 PSTI O 00 enteropesed trypelinogen chymotrypsin prophosph phospholipase Tên PSTI Figure 1. Available pancreatic enzymes, all at appropriate concentration in liquid buffer. available non-enzyme substrates whole milk (source of lipids), in liquid form o o gelatin (source of collagen protein), bound to developed film Figure 2. Available non-enzyme substrates: whole milk (left), gelatin and developed film strips (right)

available detection techniques (assays) || ₁ TELLI Figure 3. Phenol red pH indicator technique for detecting lipid digestion rate. A colour-changing pH detector, phenol red, is added to the test tube with a source of lipids and a lipase. As the lipase digests lipids, breaking the lipids down into fatty acids, the solution gradually becomes more acidic and the indicator gradually turns from pink to yellow. The faster the digestion happens, the faster the colour change happens. (Bishop et al. 2012) Figure 4. Developed film strip technique for detecting gelatin protein digestion rate. Gelatin proteins keep silver grains adhered to strips of developed photographic film, giving the film a dark grey colour. The developed film strips are immersed in a solution containing the protease. As the protein is digested by the protease, it can no longer attach the silver grains to the film, so the silver grains fall off into solution and the film strip becomes colourless. The faster the gelatin is digested, the faster the film becomes colourless. (Glider and Hargrove 2002)