Answer Happy

4. a. Calculate the ratio of your Biuret test result to the result for the Lowry test. b. Compare this ratio to 100 to 1 which approximates the difference in sensitivity of the two methods?
For each method, complete the following table and prepare the standard curve plot on the appended graph paper. Be sure to show the extrapolation of absorbance for unknown to obtain protein concentration on the graphs. Biuret Protein Standards Unknown No 12 Tube # 1 2 3 4 5 6 7 8 Absorbance 0.00 0.133 6.257 0.388 0.522 10.492 0.46% 0.4841 mg Protein/tube 2 4 6 3 7.6 7.1 Average, me protein/tube O.4813 Average, me protein /ml Calculation for amount (mg) of protein contained in tube #4 10 mg/me xo 6me: 6mg Calculation of the average concentration of protein (mg/ml) in unknown solution. reforstandard Curve so. 998 the volume of Unknow Protein in y = 0.065 +0.002 Tube - ml y=6.892+0.46870.484) -0.4913 Concentration = 7.401 mg/me 420-065x+0.002 0.4613-0.065 x + 0.0002 *= 7:401mg 3 Lowry Protein Standards Unknown No.in Tube # 1 2 3 4 5 6 7 8 9 10 Absorbance 0.00 0.05 0.150 0.24 0.257 0.1800-298 0.15 0235 0.216 mg Protein/tube 0.00 0.00 0.04 10.06 0.08 0.1 0.12 0.000 de Average, mg protein/tube Average, mg protein /ml Calculation for amount (mg) of protein contained in tube 84. 0-048 - Calculation of the average concentration of protein (mg/ml) in unknown solution R2.0.87 Concentration 0.0827 ing/me y = 2.2618X+0.029 - (0-175 +0.255 +0-210) - 0-25333 3 y = 2.2618X+ 0.029 0.215383 - 22616% +0.029 x=0.0826mg