The data in the table are used to create a calibration curve for the determination of RNA from its absorbance at 260 nm.
Posted: Sat Feb 26, 2022 11:57 am
The data in the table are used to create a calibration curve for
the determination of RNA from its absorbance
at 260 nm.
Use a spreadsheet and the least-squares method to determine the
slope and š¦y-intercept of the best straight line fit to the
calibration curve. Do not subtract the blank reading when creating
the calibration curve.
š=
Ī¼gā1
š=
Using the slope and intercept of the calibration curve,
determine the quantity of RNA in a sample that gives an absorbance
of 0.5950.595.
mass =
Ī¼g
the determination of RNA from its absorbance
at 260 nm.
Use a spreadsheet and the least-squares method to determine the
slope and š¦y-intercept of the best straight line fit to the
calibration curve. Do not subtract the blank reading when creating
the calibration curve.
š=
Ī¼gā1
š=
Using the slope and intercept of the calibration curve,
determine the quantity of RNA in a sample that gives an absorbance
of 0.5950.595.
mass =
Ī¼g