Examine fluorescence of FMN at 530 nm. Pipette various concentrations of FMN into multiwell tray provided. [1mM, 100uM,
Posted: Sun Feb 13, 2022 8:00 am
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Examine fluorescence of FMN at 530 nm. Pipette various concentrations of FMN into multiwell tray provided. [1mM, 100uM, 60 UM, 20uM, 10uM, 1ụM, 600nM, 200nM, 100nM, 10 nM, 5nM, L Load tray into microplate reader. The excitation wavelength is 450nm, the emission wavelength is 550 nm. Plot fluorescence intensity readings against [FMN] Fluorescence intensity [FMN] data: 1 2 3 4 5 6 7 8 9 10 11 144 1 1e4 9849.8 45625 25 26.3 232.84 1.484 1.2e4 9907.0 2320.4 1663.5 the 1 to 11 readings are 1mM to the 5nM concentrations draw table showing fluorescence intensity reading for each FMN conc, also draw graph of intensity versus FMN. like this for example
5. Make further dilutions of the stock FMN solution in phosphate buffer to obtain the following concentrations: 600 nM, 200 nM, 100 nM, 10 nM, and 5 nM. Line up the eleven FMN solutions with the following concentrations: 1 mM, 100 uM, 60 uM, 20 uM, 10 JM, 1 uM, 600 nM, 200 nM, 100 nM, 10nM, and 5 nM. [Dilutions from step 1 should be re-used where appropriate]. Pipette a sample of each solution into a well in the multiwell tray
will be measured using an excitation wavelength of 450 nm and an emission wavelength of 530 nm. Note the fluorescence intensity readings for each concentration. In your report, draw a table showing the fluorescence intensity reading for each concentration of FMN. Also draw graphs of intensity versus [FMN]. It may be necessary to plot two graphs to cover the range of concentrations tested.
Normalized Riboflavin fluorescence spectra 0.025 0.02 0.015 0.01 exitatio Fluorescent intensity (RFU) 0.005 emmiss o 250 350 450 550 650 750 850 Wavelenght (nm)
data: 1 2 3 4 56 7 8 9 10 11 1a 1.121 9949.8 45625 2526.3 232.84 1.484 1.264 990720 2320.4 1663.5 the 1 to 11 readings are 1mM to the 5nM concentrations draw table showing fluorescence intensity reading for each FMN conc, also draw graph of intensity versus FMN.