BUFFERS, HYFROLYSIS OF SALT
Posted: Fri Jun 10, 2022 11:28 am
BUFFERS, HYFROLYSIS OF SALT
2. Joey was tasked to ensure that an enzyme responsible for keeping his species from extinction remains biologically active. To do so, he needs to store it in a buffer solution with a pH of 10.00. Unfortunately, only three buffer systems are available in their laboratory: Buffer System Weak Acid Component Ka Conjugate Base Salt KYA Benzoic acid, C6H5COOH 6.25 x 10-5 NaC6H5COO MUH Boric acid, H3BO3 5.81 x 10-7 NaHâ‚‚BO3 TWO Hydrogen cyanide, HCN 6.20 X 10-10 NaCN A. In what buffer system (KYA, MUH, or TWO) will Joey store the enzyme? In your solution sheet, show calculations and briefly explain to support your answer. B. Write the equilibrium reaction involved in the buffer system. Write your answer on your solution sheet C. Calculate the amount (in mL) of the 1.75 M stock solution (pH 7.00) of the same buffer system needed to prepare 250 mL of a 0.550 M buffer pH 7.00.
2. Joey was tasked to ensure that an enzyme responsible for keeping his species from extinction remains biologically active. To do so, he needs to store it in a buffer solution with a pH of 10.00. Unfortunately, only three buffer systems are available in their laboratory: Buffer System Weak Acid Component Ka Conjugate Base Salt KYA Benzoic acid, C6H5COOH 6.25 x 10-5 NaC6H5COO MUH Boric acid, H3BO3 5.81 x 10-7 NaHâ‚‚BO3 TWO Hydrogen cyanide, HCN 6.20 X 10-10 NaCN A. In what buffer system (KYA, MUH, or TWO) will Joey store the enzyme? In your solution sheet, show calculations and briefly explain to support your answer. B. Write the equilibrium reaction involved in the buffer system. Write your answer on your solution sheet C. Calculate the amount (in mL) of the 1.75 M stock solution (pH 7.00) of the same buffer system needed to prepare 250 mL of a 0.550 M buffer pH 7.00.