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Fibroblast Overlay images Dictyostelium
Fibroblast Dictyostelium Tubulin Actin DAPI
Get calls - Remove media w vacuum 5. Add 1000μl para formaldehyde -Incubate in fume hood for 10 min =. Remove para- wlpipette in fume hood ・fix w/pa Add 2000μL PBS & incubate 2 min in fume hoog 7. Remove PBS & add 1000ML triton X-incubate & 5. Add 100 μL 1° antibody to parafilm 10 min 1. Flip coverslip (cells down) onto parafilm 1²3 10 0. Incubate for 45 min@ 37°C 1. Transfer coverslip back to dish & add 1000μL TBST (cells up) = Repeat TBST wash 2 more times Leave the last wo 5. Add 100 ml of 2° antibody to parafilm and transfer Coverslip (cells down) 3 + Incubate 20 min@ 37°C 5. Transfer cells to dish (cells up) & wash 3x with TBST (like step 12 Add a small drop of DAPI to slide transfer coverstip (cells down) 7. Incubate 5 min indark (drawer) & the visualize