Answer Happy

The data in the table below were collected from initial reaction rates of the mutant protease-catalysed reaction at the substrate concentrations as shown. Answer the questions that follow. vo (μM/min) [S] (mole/liter) 130 65 x 10-5 116 23 x 10-5 87 7.9 x 10-5 63 3.9 x 10-5 30 1.3 x 10-5 10 0.37 x 10-5 2.1 State two techniques that can be used to characterise the mutant HIV protease. (1 mark) 2.2 Use sound reasoning to justify why you selected the two techniques above. (2 marks) 2.3 Before you can perform enzyme activity measurements, it is necessary to identify a suitable substrate for the mutant HIV protease. Briefly discuss how you will accomplish this task. (3 marks) 2.4 The design of any enzyme assay requires knowledge of the reaction that occurs between an enzyme and a substrate. Briefly state the most important information needed when designing an enzyme assay. (3 marks)
2.5 Determine the KM and Vmax values using a suitable graphical method. You must submit your answers together with a detailed and clearly labelled graph indicating how these values were obtained. Merely reporting the KM and Vmax values are not acceptable. (4 marks) 2.6 If the original concentration of mutant HIV protease is 2.8 μmol/L, calculate the kcat (sec¹) of the mutant HIV protease. (2 marks)