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Please Interpret this gDNA Agarose gel
Method for C.
Preparation of Primer and gDNA template
Reaction components
Stock concentrations
Final reaction volume for one PCR is 25µl
In this table you are calculating volumes for 3.5
reactions
Master mix 1
Master mix 2
Final conc. or amount
µl to add
Final conc. or amount
µl to add
Primer
Concentration*
(µM)
2µM
0.2µM
10
0.4µM
20
Amount of gDNA
template (ng)
5ng/µl
25ng
5
25ng
5
OneTaq Mastermix
2X
1X
50
1X
50
Nuclease-free water
Add water such that the final total reaction volume in each tube
is 3.5 x 25µl
PLEASE LABEL WHICH RESULTS EXPLANATION A B AND C LIKE IN THE
IMAGES
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