Answer Happy

1.You stitch together the gene sequence for GFP and a gene
sequence of interest to encode a fusion protein that you express in
cultured cells. Which of the following can you do with this fusion
protein? (Select all that apply.)
choices
a. Affinity purification
b. Enzymatic cleavage of the protein of interest
c. Visualization by microscopy
d. You cannot make this fusion protein as GFP cannot be
genetically encoded
2.
The lipid bilayer forming cell membranes behaves as a 2D fluid
whose fluidity depends largely on its lipid composition.
Phospholipid molecules in the bilayer can diffuse laterally, and
the rate of diffusion as a proxy for fluidity can be observed using
FRAP experiments. Which of the following changes would lead to
longer recovery time from photobleaching?
choices
a. Adding more cholesterol to the membrane composition
b. Decreasing cis-double bond content relative to trans-double
bond content in the phospholipids
c. Increasing unsaturated phospholipid content
d. Removing membrane-embedded proteins