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Biochemistry Question
Q17-22 refer to the information below: ABCDE This is an image of a DNAse footprinting experiment testing how the small molecule IPTG effects the binding of a transcription factor to DNA. IPTG activates transcription at promoters containing the consensus sequence present in this DNA fragment. A: 76 basepair DNA fragment + DNAsel B: 76 basepair DNA fragment + DNAsel +0.5 uM transcription factor C: 76 basepair DNA fragment + DNAsel + 5 uM transcription factor D: 76 basepair DNA fragment + DNAsel +0.5 uM transcription factor + IPTG E: 76 basepair DNA fragment + DNAsel + 5 uM transcription factor + IPTG 17) Draw an additional lane next to lane A showing your predicted result if the scientist had NOT added DNAsel to lane A. (4 points)