Answer Happy

A. How does an electric field affect the migration of macromolecules? B. Why is B-mercaptoethanol (BME) used in the sample buffer (blue solution)? C. Which would you expect to migrate at a faster rate: a protein of great molecular mass or small molecular mass? Why? D. Examine the relationship between the molecular mass and R. What would happen to the R, if the gel concentration (%) increased versus decreased. E. i. What can be inferred from the protein samples from fish? ii. Do you observe relatedness based upon the protein pattern viewed by SDS-PAGE? F. i. What can you infer from the centrifugation samples, H1 and SO? ii. What obvious conclusions can be drawn from the intensity of staining pattern between the two samples? similar to this one (of course your values are different, so your line equation will be different). Standard Curve for Estimating Protein Weight 3 y+45x+25309 0.4 4 log molecular weight 25 15 1 as 0 0 0.2 Relative Mobility 12