Answer Happy

You want to probe a cell sample with a fluorophore-conjugated antibody that specifically recognizes certain proteins on the surface of the endoplasmic reticulum. How would this probe be used? a. Applied to cells in live culture and taken in through the plasma membrane. O b. Applied to dead cells that have been fixed and permeabilized cells. O c. Expressed on a plasmid in these cells. O d. Packaged in a virus for stable transduction.
You stitch together the gene sequence for GFP and a gene sequence of interest to encode a fusion protein that you express in cultured cells. Which of the following can you do with this fusion protein? (Select all that apply.) a. Affinity purification b. Enzymatic cleavage of the protein of interest c. Visualization by microscopy Od. You cannot make this fusion protein as GFP cannot be genetically encoded