ou are studying a species of finch in the Galápagos Islands, and you have identified a gene that controls how finch beak
Posted: Thu Jun 09, 2022 12:50 pm
ou are studying a species of finch in the Galápagos Islands, and you have identified a gene that controls how finch beaks develop. This gene has three different alleles in the population that are distinguished by the presence or absence of a restriction fragment length polymorphism (RFLP) site indicated with the down arrow". Location of PCR primer binding sites is indicated as well. a.) You perform PCR, a restriction enzyme digest, and separate the different RFLP patterns out on a gel. Using the A/B and A/C heterozygous gel results and the provided patterns, draw in the bands you would expect to observe for each homozygous pattern and the heterozygous B/C pattern. pattern i B From your studies you have also come to the following conclusions: i.) Pattern A is a functional wild type allele. ii.) Patterns B and C are hypomorph mutations. iii.) You never observe adult finches that have a C/C genotype. You only observe C/C individuals when you genotype finch eggs that start embryogenesis, but never hatch. A/B finches have wild type beaks, but A/C and B/C finches have mutant beaks which have a different mutant phenotype than B/B mutant finches. iv.) b.) In the diagram above, circle the RFLP site(s) that are linked with the mutant phenotype(s). can explain why C/C adult finches are never observed. c.). d.) (1 pt.) Propose an explanation as to why B/C and B/B finches have different mutant phenotypes. AVA B/B A/B A/C " || | B/C