Answer Happy

The table shows where different restriction endonucleases (restriction enzymes) cleave DNA. The abbreviation R represents the purines (adenine and guanine). The pyrimidines (cytosine, thymine, and uracil) are abbreviated as Y. The abbreviation W represents adenine or thymine. Enzyme Target sequence Cleavage 5' GAATTC 3' EcoRI 5'G AATTC 3' 3' CTTAAG 5' 3' CTTAA G5' 5' GATATC 3' EcoRV 5' GAT 3' CTATAG S' 3' CTA 5' GGCC 3' 5' GG HaelII 3' CCGG 5' 3' CC 5' AAGCTT 3' S'A AGCTT 3' HindIII 3' TTCGAA 5' 3' TTCGA AS' S'RG PpuMI 5' RGGWCCY 3' 3' YCCWGGR 5' GWCCY 3' GR S' 3' YCCWG Which restriction endonucleases would cleave a DNA molecule at the given sequences? The complementary DNA substrate strand is omitted for clarity. 5' AGGCCGAATTCGC 3' 5' TCAGAATTCGGTGA 3' Haelll HaeIII EcoRI EcoRV Hind!!! EcoRI EcoRV HindIII ATC 3' TAG 5' CC 3' GG 5'