Answer Happy

second position с U G 4. Genome Decoding: Replication, Transcription, Translation and Targeting - Practical Application (20 points total) The amino acid sequence of a short neural peptide is as follows: MGYTGASD. To produce a large quantity of the peptide using E. coli, the gene encoding the short peptide is cloned into a plasmid vector that can be replicated in E coli using the E coli OriC as the origin of replication. Using the genetic code table, we can find a DNA sequence to encode the peptide (many sequences will give the same peptide sequence due to the degeneracy of the genetic codes). (a) (5 pts) Show one possible DNA sequence that can be used to encode the peptide. UAU UAC Tyr U UAA stop UAG stop CAU His CAC CAA CAG Gin (b) (10 pts) In addition to the coding sequence, what other essential sequences/elements are required for the transcription and translation of the peptide in E coli (a prokaryote)? Use a simple diagram to illustrate all the necessary elements/sequences. AAU Asn AAC AAA Lys AAG (c) (5 pts) If a signal peptide sequence is attached to the N-terminus of the peptide, where do you think the peptide will end up in (the growth GAU Asp GAC GAA Glu medium/periplasmic space, or the cytosol of E coli)? Explain your answer. GAG first position (5' end) C A G UUU UUC UUA UUG CUU CUC CUA CUG AUU AUC lle AUA AUG Met GUU GUC Val GUA GUG Phe Leu Leu UCU UCC UCA UCG CCU CCC CCA CCG ACU ACC ACA ACG GCU GCC GCA GCG Ser Pro Thr Ala A DUA DUA UGU Cys UGC UGA stop А UGG Trp G CGU CGC Arg CGA CGG AGU Ser AGC AGA Arg AGG GGU GGC Gly GGA GGG DUA DU AG G third position (3' end)