An actively respiring bacterial culture is supplied with pyruvate labeled on the methyl carbon with carbon-14 (carbon-14
Posted: Thu Jun 09, 2022 11:03 am
An actively respiring bacterial culture is supplied with
pyruvate labeled on the methyl carbon with carbon-14 (carbon-14 is
radioactive). Shortly after adding the label the citric acid
intermediates are isolated and the position of the carbon-14 atom
is determined.
i) Where is the carbon-14 label on alpha-ketoglutarate? Consider
only the initial incorporation of carbon-14 in the first pass of
labeled pyruvate through the citric acid cycle. Please draw the
relevant structures that display how pyruvate is transformed to
alpha-ketoglutarate and provide balanced equations.
ii) What enzyme(s) in the pathway that convert pyruvate to
alpha-ketoglutarate are regulated?
pyruvate labeled on the methyl carbon with carbon-14 (carbon-14 is
radioactive). Shortly after adding the label the citric acid
intermediates are isolated and the position of the carbon-14 atom
is determined.
i) Where is the carbon-14 label on alpha-ketoglutarate? Consider
only the initial incorporation of carbon-14 in the first pass of
labeled pyruvate through the citric acid cycle. Please draw the
relevant structures that display how pyruvate is transformed to
alpha-ketoglutarate and provide balanced equations.
ii) What enzyme(s) in the pathway that convert pyruvate to
alpha-ketoglutarate are regulated?