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Question 3 [9 marks] The activity of an enzyme may be studied by following the rate of substrate depletion or product formation during an enzyme-catalysed reaction. There are two types of assays that are used to monitor the rate of an enzyme-catalysed reaction. These are: 1) Kinetic assay and 2) Fixed-time assay. The figure below represents the kinetics progress curve of an enzyme- catalysed reaction. 24+ Kinetic assay 20+ Fixed-time assay 16+ 12+ End of fixed-time assay 3 6 7 μmoles m 4 1 2 4 5 Time (min) 8
Study the plots in the figure above and provide a critical analysis of the two assays. (9 marks)