The enzyme examase catalyses the following reaction, A → B where A is the reactant and B is the product A595 measurement
Posted: Thu Jun 09, 2022 10:48 am
The enzyme examase catalyses the following reaction, A → B
where A is the reactant and B is the product
A595 measurements were used to determine the catalytic activity
of examase by measuring the rate at which B is produced. From this,
the activity of the enzyme was determined to be 12 µmol/mL per
minute.
A standard albumin solution (concentration = 10 mg/mL) was used
to prepare a standard curve of Abs595 vs amount of protein in mg.
The protein assay was also performed using 100 uL of a 1 in 20
dilution of the original examase sample. For the absorbance
measurements, it was determined that the sample used in the A595
measurement contains 1.5 mg of examase.
Which of the following statements correctly gives the specific
activity of examase?
Group of answer choices 0.4 U/mg 24 U/mg 240 U/mg 0.04 U/mg 4
U/mg
where A is the reactant and B is the product
A595 measurements were used to determine the catalytic activity
of examase by measuring the rate at which B is produced. From this,
the activity of the enzyme was determined to be 12 µmol/mL per
minute.
A standard albumin solution (concentration = 10 mg/mL) was used
to prepare a standard curve of Abs595 vs amount of protein in mg.
The protein assay was also performed using 100 uL of a 1 in 20
dilution of the original examase sample. For the absorbance
measurements, it was determined that the sample used in the A595
measurement contains 1.5 mg of examase.
Which of the following statements correctly gives the specific
activity of examase?
Group of answer choices 0.4 U/mg 24 U/mg 240 U/mg 0.04 U/mg 4
U/mg