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Question # 2 An enzyme catalyzed reaction exhibits simple Michaelis- Menten kinetics (Vo = (Vmax*S)/(Km+S) ). The graph on the right shows a double reciprocal plot of substrate concentration (1/[S]) versus initial velocity (1/Vo) for the enzyme. The total enzyme concentration [ET] = 2 µM. [In your answers make sure to include the appropriate units] 2A Calculate the Km value of the enzyme. 2B Calculate the turn-over number of the enzyme. -0.4 2D You repeat the experiment, but this time in the presence of a competitive inhibitor. The dissociation constant of the inhibitor (K₁') is 4 x 10-⁹M and the inhibitor concentration is 8 x 10-ºM. On the plot, graph the expected 1/S vs. 1/Vo behavior of the enzyme in the presence of the uncompetitive inhibitor. 2E In the presence of the inhibitor what is the apparent maximal velocity of the enzyme? Sea 2F Which form of the enzyme does the inhibitor bind? [[ (E) or (ES) or (ES and E) ]] (circle your answer) Question #3 Complex IV (cytochrome oxidase) in the electron transport chain transfers electrons from reduced Cytrochrome C to molecular oxygen. 3A. Write a balanced equation for the overall redox reaction performed by complex IV. Indicate the electron donor and acceptor in the redox reaction. [Do not include the intermediates in this overall reaction. Do not account for ton pumping]. 3B. Calculate the change in the standard reduction potential for this reaction. [see table on page 2]. 3C Which has a lower amount of free energy at the standard state, the products or the reactants? 3 (sec/10 M) -0.2 0.4 0.2 (10 M) 0.2 1 [$] 0.4