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Reaction Kinetics Worksheet Questions 1. What does the following mean? A-1₁-12 P 2. Are the following reactions First-order, second order, or Pseudo-first order? Explain [2] Q-R Q+S Q+QR R Q+S-R ([Q] >> [S]) 3. Provide an equation for the instantaneous velocity for Q+S-R [2] 4. Consider the nonenzymatic elementary reaction AB. When the concentration of A is 20 mM, the reaction velocity is measured as 5 μM B produced per minute. (a) Calculate the rate constant for this reaction. (b) What is the molecularity of the reaction [4] 5. Explain the following: 5.1 What is Zero order? 5.2 What is Steady-state? [2] 6. Explain what the "turn-over number is? [2] 7. What is the lineweaver-burk plot used for? [2] [2] 8. Explain how drawing a double-reciprocal plot can be used to calculate Vmax and Kv 9. At what concentration of S (expressed as a multiple of Ku) will Vo=0.95 Vmax? [3] 10. Calculate KM and Vmax from the following data: [S] (µm) Vo(mm/s) 0.34 0.1 0.53 0.2 0.74 0.4 0.91 0.8 1.04 1.6 [6] 11. Define bisubstrate, sequencial and ping-pong reactions 12. Is it necessary to know [E]r in order to determine (a) KM, (b) Vmax, or (c) Keat? [3] [3]