Answer Happy

Based on the diagram below answer the following questions: Organon hole Tetrahedral intermediate Jo Lo Oryanion hole 97 Tetrahedral intermediate 0 jo O Ⓒ Acyl-enzyme 16,0 Acyl-enzyme

Tetrahedral Intermediate Ay-y a) What enzyme mechanism is depicted in the figure above? [Select] b) What are the names of the three amino acids in the catalytic site, in the correct order from left to right [Select] c) How does histidine from the catalytic triad act in step 2? [ Select] d) How does histidine from the catalytic triad act in step 3? [Select] ✓? e) Which amino acid acts as a nucleophile [Select] f) Which steps of the mechanism above correspond to the burst phase, and which steps correspond to the steady- state phase, as shown in the graph below? [Select]

f) Which steps of the mechanism above correspond to the burst phase, and which steps correspon state phase, as shown in the graph below? [Select] Steady-state phase Absorbance (p-nitrophenol released) - Burst phase Milliseconds after mixing -

Tetrahedral intermediate a) What enzyme mechanism is depicted in the figure above ✓ [Select] b) What are the names of the three amino acids in the catal Trypsin Chymotrypsin Zymogen [Select] c) How does histidine from the catalytic triad act in step 2? [Select] d) How does histidine from the catalytic triad act in step 3? [Select] ? e) Which amino acid acts as a nucleophile [Select] Acyl-entyme m left to right and to the's

Tetrahedral Intermediate a) What enzyme mechanism is depicted in the figure above? [Select] b) What are the names of the three amino acids in the catalytic site, in the co ✓ [Select] histidine, asparagine, serine aspartate, histidine, serine tyrosine, histidine, serine catalytic triad act in step 2? [Select] d) How does histidine from the catalytic triad act in step 3? [Select] e) Which amino acid acts as a nucleophile [Select]

What are the names of the three amino acids in the catalytic site, in the correct order from left to right Felect] w does histidine from the catalytic triad act in step 2 ✓ [Select] histidine acts as a base histidine acts as an acid Tociety w does histidine from the catalytic triad act in step 3 ch amino acid acts as a nucleophile [Select] h steps of the mechanism above correspond to the burst phase, and which steps correspond to the mase, as shown in the graph below? [Select]

[Select] c) How does histidine from the catalytic triad act in step 2? [Select] d) How does histidine from the catalytic triad act in step 3 ✓ [Select] histidine acts as a base histidine acts as an acid e) Which amino acid acts as a nucleophile [Select] f) Which steps of the mechanism above correspond to the burst phase, and which steps correspond to the ste state phase, as shown in the graph below? [Select] Steady-state phase ed) →

d) How does histidine from the catalytic triad act in step 3? [Select] e) Which amino acid acts as a nucleophil✓ [Select] ? asparagine aspartate f) Which steps of the mechanism above nd which steps correspond to serine state phase, as shown in the graph below histidine tyrosine Steady-state phase Absorbance Ophenol released) → Burst phase

d) How does histidine from the catalytic triad act in step 3? [Select] e) Which amino acid acts as a nucleophile [Select] f) Which steps of the mechanism above correspond to the burst phase, and which steps correspond to the steady- state phase, as shown in the graph below ✓ [Select] burst phase: steps 1-2; steady state: steps 6-8 burst phase: steps 1-4; steady state: steps 5-8 burst phase: steps 1-6; steady state: steps 7-8 Steady-state phase Absorbance nitrophenol released) Burst phase