Answer Happy

Post Lab Questions 1. 2. Did acetone denature the protein in this laboratory experiment (Tube 437 b. Draw expanded structures of both an acetone molecule and a water molecule and then use a dash line to represent the Hydrogen Bonding that can occur between these two substances 2. Changes in pH will denature a protein (Tubes 8 and 9) by changing the net charge on the protein. This is possible because proteins have ionizable groups in their structures (namely amine functions and carboxyl functions) whose charged state depends on the pH of the solution the protein is in. Salt Bridges in the protein will be destroyed by changes in pH. Shown the change that will occur for each of the following that will destroy the 'salt bridge in the diagram: addition of OH H₂N (CH₂R addition of H eco HNCHER 3. The compound urca has been used for decades as a donaturant in the study of protein stability. Draw an expanded structure of urca and circle the functional groups that make it such a strong hydrogen bonder: 4. Yeast is used as the leavening agent in bread making. The yeast metabolizes the natural carbohydrates in the flour, converting them to CO, and ethyl alcohol and it is the CO. gas that raises the bread. So, when making yeast bread it is very important that the mixture not be overheated during the leavening process because if it is overbeated the bread will not raise. Explain in terms of protein denaturation: 5. In its native conformation, phycocyanin has a max of 620 nm. What is the color of the light being absorbed at this wavelength? (You can find the answer on the Table from last weeks lab!) color: